When it comes to blood testing, the quality of the sample is critical for reliable results. The 10ml EDTA Tube with Purple Top is a standard blood collection tube used widely in hospitals and labs to collect and preserve blood for hematology and molecular testing.

What Is a 10ml EDTA Tube with Purple Top?

This tube is a vacuum-sealed container pre-coated with EDTA (Ethylenediaminetetraacetic acid), an anticoagulant that binds calcium ions in blood, preventing it from clotting. The purple (or lavender) colored cap indicates it contains EDTA, helping healthcare workers quickly identify its use.

The 10ml size allows for a larger volume of blood collection, suitable for multiple or detailed analyses from a single sample.

Why Is EDTA Used?

Blood clotting begins as soon as blood leaves the body, which can affect many laboratory tests. EDTA chelates calcium, a crucial factor for coagulation, preventing clot formation. This keeps blood cells intact and maintains plasma in a liquid form, essential for accurate blood counts and molecular tests.

Main Uses of the 10ml EDTA Purple Top Tube

• Complete Blood Count (CBC): Assessing red cells, white cells, and platelets.

• Blood Smears: For microscopic evaluation of blood cell shape and structure.

• Molecular Testing: Preserving DNA/RNA for genetic and infectious disease testing.

• Immunophenotyping: Identification of cell types through flow cytometry.

• Blood Typing: Critical in transfusion medicine.

• Research: Larger sample size supports extensive or multiple tests.

Collection and Handling Guidelines

• Fill to 10ml Mark: Proper fill volume ensures accurate anticoagulant-to-blood ratio.

• Mix Gently: Invert the tube 8-10 times immediately after collection to mix EDTA evenly.

• Avoid Vigorous Shaking: Prevents hemolysis and damage to blood cells.

• Process Quickly: Ideally, test the sample within 6 hours.

• Store Properly: Keep at room temperature; avoid freezing or overheating.

Benefits of the 10ml EDTA Tube

• Efficient anticoagulation without damaging blood cells.

• Larger volume allows for multiple tests from a single draw.

• Standardized purple cap reduces risk of collection errors.

• Compatible with automated laboratory equipment.

• Ensures sample stability for hematology and molecular diagnostics.

Limitations

• Not suitable for clotting tests (e.g., PT, aPTT).

• EDTA can affect calcium and potassium level measurements.

• Incorrect filling volume can cause test inaccuracies.

Conclusion

The 10ml EDTA Tube with Purple Top is a vital tool for accurate blood testing in clinical laboratories. Its anticoagulant properties preserve the blood sample's integrity, allowing for precise hematology and molecular diagnostics. Proper collection and handling are essential to maximize its effectiveness and ensure trustworthy results for patient care.